ABSTRACT. – Coleomegilla maculata, Cycloneda sanguinea and Eriopis connexa (Coleoptera: Coccinellidae) are important predators of agricultural pests. Chlorpyrifos (Nufos®480CE, O,Odiethyl O-3,5,6-trichloropyridin-2-yl phosphorothioate) was dissolved in distilled water at 5.84mL/3L, and was added to extracts of native plants from Brazilian Cerrado, a Savannah-type biome, including Ageratum fastigiatum (Asteraceae), Campomanesia adamantium (Myrtaceae), Eremanthus erythropappus (Asteraceae), Jacaranda caroba (Bignoniaceae) and Pseudobrickellia brasiliensis (Asteraceae) in dimethylsulfoxide [DMSO, (CH3)2SO] at 20g/L. These solutions were sprayed at 600mL/ha in an experimental rolling mat of Petri dishes lined with filter paper. Four-day-old adults of C. maculata, C. sanguinea and E. connexa were kept singly in these Petri dishes in the first day and replaced by another individual of the same species after 1, 2, 3, 4, 5, and 6 days of the spraying of insecticides. The mortality of individuals in the first day to fifth day after spraying with chlorpyrifos was 93.78% and in the first 3 days after spraying with plant extracts was 68.59%. The residual effect of chlorpyrifos caused high mortality of C. maculata, C. sanguinea and E. connexa until the fifth day of its application. The mortality of these insects was reduced in the fourth day after treatment with botanical extracts. Extracts from leaves and flowers of A. fastigiatum should be preferred for integrated pest management (IP M) programs with Coccinellidae predators.